Figure 2.

Kinetic parameters V_max and k_cat of CK1δ wild type and mutants with different substrates. Michaelis–Menten kinetics have been analyzed for CK1δ wild type and mutants using either α-casein, GST-β-catenin¹⁻¹⁸¹, or GST-p53¹⁻⁶⁴ as substrate. The kinetic parameters V_max (a) and k_cat (b) were normalized toward the respective parameters determined for CK1δ wild type. Data is presented as mean values and standard deviation (SD) for experiments performed in triplicate. Abbreviations: A, alanine; E, glutamic acid; G, glycine; GST, glutathione S-transferase; H, histidine; I, isoleucine; K, lysine; k_cat, turnover number; L, leucine; M, methionine; P, proline; Q, glutamine; R, arginine; S, serine; V, valine; V_max, maximum enzyme reaction velocity; W, tryptophan; WT, wild type; Y, tyrosine; *, stop codon.