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. 2019 Dec 7;20(24):6184. doi: 10.3390/ijms20246184

Figure 6.

Figure 6

Compounds 18 and 19 show CK1δ-specific effects on cell viability. (a) MTT viability assays have been performed by treating parental HeLa cells, HeLa CK1δ−/−, or HeLa CK1δ+/+ cells with 25 μM of compounds 16, 17, 18, 19, or DMSO as control for 48 h. Cell viability of treated cells has been normalized toward the viability of DMSO-treated cells. Results are presented as mean values of experiments performed in triplicate. Error bars represent the SD. Statistical analysis was done by performing two-way ANOVA using following levels of significance: * p ≤ 0.05; **** p ≤ 0.0001; ns, not significant. (b) EC50 values were determined by treating HeLa CK1δ−/− and HeLa CK1δ+/+ cells with compound 18 in a concentration range between 0.313 and 70 µM for 48 h. Cell viability of treated cells has been normalized toward the viability of DMSO-treated cells. Results are presented as mean values of experiments performed in triplicate. Error bars represent the SD. EC50 values have been calculated using GraphPad Prism 6. Abbreviations: EC50, 50% effective concentration; µM, micromolar.