Figure 8.

Compounds 20, 21, and 22 significantly inhibit kinase activity of CK1δ and ε. (a) Kinase activity of CK1α, γ, δ, and ε was analyzed in presence of 10 µM of newly designed IWP-derivatives 20, 21, and 22. Results are presented as mean values of experiments performed in triplicate. Error bars indicate the standard error. (b) In vitro IC₅₀ values were determined for CK1δ and a dilution series of compounds 20, 21, and 22. IC₅₀ values were calculated using GraphPad Prism 6. Results are presented as mean values ± standard error of experiments performed in triplicate. (c) Structures of inhibitor compounds 20, 21, and 22. Abbreviations: DMSO, dimethyl sulfoxide; IC₅₀; 50% inhibitory concentration; µM, micromolar.