Figure 6.

Role of gibberellic acid in the delayed embryo development of Atcrk5-1. (A) Quantification of embryo axis length differences in siliques S11–S12. Embryo axis from SAM-to-RAM was measured after the treatment of developing inflorescences of wild type (Col-0) and Atcrk5-1 mutant plants without (GA −) or with 20 μM GA₃ (GA +). The exogenous GA₃ supply restored the length of the mutant embryos to the wild type level. (Student’s t-test:* p < 0.05, n = 30). The data presented are the means of two biological repeats. (B) Quantification of the total GA content in medium stage siliques (S5–S6–S7). 30-30 siliques were collected from wild type (Col-0) and mutant (Atcrk5-1) inflorescence. The total GA content was determined using a GA-antibody-based Elisa kit. (C) Rescue of the delayed embryo bending phenotype in S11 of the Atcrk5-1 mutant by 20 μM GA₃ treatment with its noticeable change from walking stick to bent cotyledon stage, while Col-0 remains in the bent cotyledon stage. Representative images are shown from experiments repeated two times with 30-30 mutant and wild type embryos. Scale bars = 100 µm.