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. 2019 Dec 4;20(24):6120. doi: 10.3390/ijms20246120

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Relative expression level of selected genes involved in Gibberellic Acid (GA) metabolism and signaling at different silique stages quantified by qRT-PCR. The relative transcript levels were determined in small siliques (S; S1–S4, 3DAP) and medium siliques (M, S5–S7, 5DAP) for the DELLA GA signaling genes (A) RGL1, (B) RGL2, (C) RGL3, (D) GA-INSENSITIVE (GAI), and (E) REPRESSOR OF ga1-3 (RGA), the gibberellin biosynthesis genes (F) GA20ox2 and (G) GA20ox3, and the gene of the GA catabolism enzyme (H) GA2ox4. Ubiquitin1 (UBQ-1) was used as an internal control. The values are means (+/−) SD of two independent biological repeats. Student’s t-test: * p < 0.05, ** p < 0.01, *** p < 0.001. Primers used are listed in the Table S1.