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. Author manuscript; available in PMC: 2020 Jan 3.
Published in final edited form as: J Mol Diagn. 2017 Mar 21;19(3):341–365. doi: 10.1016/j.jmoldx.2017.01.011

Figure 2.

Figure 2

Determining depth of sequence. Given an allele burden of 5% and 250 read depth, the binomial distribution of true positives (TPs) can be calculated. Also, given a sequence error rate of 1%, the binomial distribution of false-positive (FP) results can also be calculated and shown to overlap the true positive distribution. The overlap of true-positive and false-positive distributions should be considered when determining minimum depth of sequence needed to reliably detect a given allele burden.