Table 2.
Composition and Osmolality Values of 8 Candidate Frozen Liquid Formulations for Each NRRV Antigen for Their Individual Bulk Storage
| F # | Formulation Components (pH 7.2) | Osmolality (mOsm) |
|---|---|---|
| 1 | 1 mM sodium phosphate +150 mM NaCl (current bulk formulation) | 270 ± 1 |
| 2 | 1 mM sodium phosphate +150 mM NaCl +0.025% PS-80 | 268 ± 1 |
| 3 | 1 mM sodium phosphate +10% w/v sucrose +0.025% PS-80 | 328 ± 2 |
| 4 | 5 mM sodium phosphate +150 mM NaCl +0.025% PS-80 | 297 ± 4 |
| 5 | 5 mM sodium phosphate +10% w/v sucrose +0.025% PS-80 | 330 ± 8 |
| 6 | 1 mM sodium phosphate +7.5% w/v sucrose +50 mM NaCl +0.025% PS-80 | 343 ± 4 |
| 7 | 5 mM sodium phosphate +7.5% w/v sucrose +50 mM NaCl +0.025% PS-80 | 308 ± 4 |
| 8 | 5 mM HEPES +10% w/v sucrose +0.025% PS-80 | 308 ± 4 |
| 9 | 5 mM HEPES +7.5% w/v sucrose +50 mM NaCl +0.025% PS-80 | 331 ± 2 |
Osmolality values are from triplicate measurements and error bars represent 1 SD.