Skip to main content
. 2019 Dec 24;29(13):4646–4656.e4. doi: 10.1016/j.celrep.2019.11.111

Figure 2.

Figure 2

In Vitro Reconstitution of the Human Nigro-striatal Circuitry in a Microfluidic Device

(A) Illustration of the nigro-striatal pathway in the brain. Substantia nigra (SN) DANs project to the dorsal striatum (Str) to connect with the medium spiny neurons (MSNs).

(B) Schematic design of the three-compartment microfluidic device with the DA and striatal lateral chambers connected via microgrooves with the central synaptic channel (SC).

(C–H) Representative images of TH+ DANs seeded in the microfluidic device.

(I–K) Representative images of DARPP32+ MSNs seeded in the microfluidic device.

(L) Quantification of DANs and MSNs relative to respectively the GFP- or tdTomato-transduced cells, after 6 weeks of culture in the microfluidic platform.

Data are mean ± SEM; n = 3 independent experiments. Scale bars, 100 μm.