Figure 1.

Viral dsRNA Mimic Triggers Formation of Persistent SGs

(A) Timelines for the experiments to study SG persistence in SH-SY5Y cells. Time points for treatments or transfection, wash-off, and cell collection are indicated by red, blue, and pink arrowheads, respectively.

(B) Representative images and quantification of SG-containing cells after stress and recovery. The percentage of SG-positive cells at each time point is indicated on the scatterplots. The x axis labeling (1–4) corresponds to the column labeling (1^st stress, 1^st recovery, 2^nd stress, and 2^nd recovery). SGs were visualized using anti-G3BP1 staining. Between 250 and 300 cells were analyzed per time point for each stressor. N/A, not available; single application of poly(I:C) was used. Data are represented as mean ± SEM; mean percentages of cells with SGs are also indicated on the scatterplots. Scale bar, 10 μm.