REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-eIF2α (phosphorylated, Ser51)	Abcam	Cat#ab32157; RRID:AB_732117
Rabbit polyclonal anti-ULK1	Abcam	Cat#ab240916
Mouse monoclonal anti-G3BP1	BD Biosciences	Cat#611126; RRID:AB_398437
Mouse monoclonal anti-TIAR	BD Biosciences	Cat#610352; RRID:AB_397742
Rabbit polyclonal anti-IFNAR1	Bethyl	Cat#A304-290A; RRID:AB_2620486
Rabbit polyclonal anti-OPTN	Bethyl	Cat#A301-829A; RRID:AB_1264331
Rabbit polyclonal anti-TBK1	Bethyl	Cat#A300-093A; RRID:AB_2303002
Rabbit polyclonal anti-cleaved caspase 3	Cell Signaling	Cat#9661S; RRID:AB_2341188
Rabbit monoclonal anti-eIF2α (total)	Cell Signaling	Cat#D7D3; RRID:AB_10692650
Mouse monoclonal anti-puromycin (clone 12D10)	Merck Millipore	Cat#MABE343; RRID:AB_2566826
Rabbit polyclonal anti-FUS	Proteintech	Cat#11570-1-AP; RRID: AB_2247082
Rabbit polyclonal anti-Nup107	Proteintech	Cat#19217-1-AP; RRID:AB_10597702
Rabbit polyclonal anti-Nup98-Nup96	Proteintech	Cat#12329-1-AP; RRID:AB_10973678
Rabbit polyclonal anti-YBX1	Proteintech	Cat#20339-1-AP; RRID:AB_10665424
Rabbit polyclonal anti-ATXN2	Proteintech	Cat#21776-1-AP; RRID:AB_10858483
Rabbit polyclonal anti-KPNA2	Proteintech	Cat#10819-1-AP; RRID:AB_2265526
Rabbit polyclonal anti-TNPO1	Proteintech	Cat#20679-1-AP; RRID:AB_10694291
Mouse monoclonal anti-FUS	Santa Cruz	Cat#sc-47711; RRID:AB_2105208
Rabbit polyclonal anti-βIII-tubulin (Tuj)	Sigma-Aldrich	Cat#T2200; RRID:AB_262133
Mouse monoclonal anti-β-actin	Sigma-Aldrich	Cat#A5441; RRID:AB_476744
Bacterial and Virus Strains
NEB 5-alpha Competent E. coli	New England Biolabs	Cat#C2987
Respiratory syncytial virus (RSV) wild-type strain A2	Openshaw Lab 18287232 (first used in this study)	N/A
Biological Samples
Spinal cord tissue from ALS patients and healthy individuals (frozen)	Sheffield Brain Tissue Bank	Request No.15-011
Spinal cord tissue from ALS patients and healthy individuals (paraffin blocks)	MRC London Neurodegenerative Diseases Brain Bank	Request No.1470
Chemicals, Peptides, and Recombinant Proteins
MG132 (proteasome inhibitor)	Calbiochem	Cat#474790
Pifithrin-μ (HSP70 inhibitor)	Enzo Life Sciences	Cat#BML-AP503
Polyinosinic–polycytidylic acid potassium salt, poly(I:C)	Sigma-Aldrich	Cat#P9582
Sodium arsenite	Sigma-Aldrich	Cat#35000
Dithiothreitol (DTT)	Sigma-Aldrich	Cat#43815
Interferon beta-1a	Sigma-Aldrich	Cat#IF014
Cycloheximide	Sigma-Aldrich	Cat#C7698
Puromycin dihydrochloride	Sigma-Aldrich	Cat#P8833
5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB)	Sigma-Aldrich	Cat#D1916
Actinomycin D	Sigma-Aldrich	Cat#A1410
Critical Commercial Assays
GenElute™ Total RNA Purification Kit	Sigma-Aldrich	Cat#RNB 100
RNase free DNase set	QIAGEN	Cat#79254
USB® Poly(A) Tail-Length Assay Kit	Thermo Scientific	Cat#764551KT
FuGENE® HD transfection reagent	Promega	Cat#E2311
DreamTaq HS polymerase	Thermo Scientific	Cat#EP2702
SuperScript IV Reverse Transcriptase	Thermo Scientific	Cat# 18090010
Lipofectamine 2000 transfection reagent	Thermo Scientific	Cat#11668027
Experimental Models: Cell Lines
Human: SH-SY5Y neuroblastoma cells (ATCC)	Sigma-Aldrich	Cat#94030304
Human: SH-SY5Y FUS line: ΔNLS1_ho (homozygous)	An et al., 2019	N/A
Human: SH-SY5Y FUS line: ΔNLS4_ho (homozygous)	An et al., 2019	N/A
Human: SH-SY5Y FUS line: ΔNLS7_ho (homozygous)	An et al., 2019	N/A
Human: SH-SY5Y FUS line: ΔNLS2_het (heterozygous, FUS granule positive)	An et al., 2019	N/A
Human: SH-SY5Y FUS line: ΔNLS8_het (heterozygous)	An et al., 2019	N/A
Human: SH-SY5Y FUS line: ΔNLS10_het (heterozygous)	An et al., 2019	N/A
Human: SH-SY5Y FUS line: ΔNLS11_het (heterozygous, FUS granule positive)	An et al., 2019	N/A
Human: SH-SY5Y FUS line: FUS knockout line	An et al., 2019	N/A
Human: primary fibroblasts (healthy control and bearing FUS P525L mutation)	Lo Bello et al., 2017	N/A
Human: Day 40 motor neurons derived from hES H9 cells	Shelkovnikova et al., 2017	N/A
Oligonucleotides
Primers for qRT-PCR, see Table S1	An et al., 2019 and this paper	N/A
Primer: Poly(A) tail assay for FUS, P1: 5′-GTCCAGCCCA TGTGAGACTT-3′	This paper	N/A
Primer: Poly(A) tail assay for FUS, P2: 5′-AACCTCCAGC ATAAAAGGGCT-3′	This paper	N/A
siRNA, targeting human STAT1: MISSION® esiRNA	Sigma-Aldrich	Cat#EHU010121
siRNA, scrambled: AllStars Negative Control siRNA	QIAGEN	Cat#SI03650318
Recombinant DNA
Plasmid: FUS R522G GFP	Shelkovnikova et al., 2014a	N/A
Software and Algorithms
CellF software	Olympus	N/A
Zen software	Zeiss	https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
Adobe Photoshop CS3	Adobe Inc.	https://www.adobe.com/uk/products/photoshop.html
GraphPad Prism 6	GraphPad Software, Inc.	https://www.graphpad.com/scientific-software/prism/
Leica Application Suite AF software	Leica Microsystems	https://www.leica-microsystems.com/products/microscope-software/p/leica-application-suite/
Other
BX61 microscope equipped with F-View II camera	Olympus	https://www.olympus-ims.com/en/microscope/bx61-2/
LSM880 microscope	Zeiss	https://www.zeiss.com/microscopy/int/products/confocal-microscopes.html
EVOS XL Core system	Thermo Scientific	Cat#AMEX1100
Leica TCS SP2 MP confocal microscope	Leica Microsystems	https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-tcs-sp2/
StepOnePlus™ Real-Time PCR System	Thermo Scientific	Cat#4376600