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. 2020 Jan 2;39:4. doi: 10.1186/s13046-019-1512-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — AMSC-Exo-mediated miR-199a-3p transfer into HCC cells. a Real-time PCR detection of miR-199a-3p expression in navie AMSCs and miRNA-modified AMSCs. b Transmission electron microscopy detection on AMSC-Exo morphology and nanoparticle tracking analysis on exosome particle size and concentration (Scale bar = 0.5 μm). c Flow cytometric analysis of the surface markers of the exosomes derived from navie AMSCs and miRNA-modified AMSCs. d Real-time PCR detection of miR-199a-3p expression in AMSC-Exo. e Real time-PCR detection of miR-199a-3p expression in AMSC-Exo-treated PLC/PRF/5 cells. f Confocal images of AMSC-199a stained with DilC₁₆(3) and the PLC/PRF/5 cells incubated by the exosomes derived from DilC₁₆(3)-stained AMSC-199a. Data are presented as mean ± S.D. (**P < 0.01, n = 3)