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. Author manuscript; available in PMC: 2020 Jan 3.
Published in final edited form as: Biochemistry. 2019 Aug 26;58(36):3813–3822. doi: 10.1021/acs.biochem.9b00523

Figure 3.

Figure 3.

(A and B) Fluorescence images of MRSA COL PBP4-YFP show that PBP4 primarily localizes at the division septum of dividing cells. (C and D) BPEI delocalizes PBP4 from the division septum. (E) Quantitative analysis of the fluorescence intensity at the division septum vs fluorescence intensity at peripheral cell wall regions was performed for untreated and BPEI-treated samples. Analysis was performed for >100 cells of each sample that showed a visible closed septum. The PBP4 protein in untreated cells (S/L = 2.95 ± 0.06) localized more at the division septum than in the BPEI-treated samples (S/L = 2.15 ± 0.05). The scale bar is 1 μm.