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. Author manuscript; available in PMC: 2020 Jul 18.
Published in final edited form as: Methods Enzymol. 2019 Jul 18;626:447–474. doi: 10.1016/bs.mie.2019.06.028

Figure 2.

Figure 2.

The purification scheme for zCD2 utilizes a HisTrap™ HP column, off-column digestion with 6 mg/ml TEV protease, a second HisTrap™ HP column for further purification, followed by a HiLoad™ Superdex™ 26/600 200 column.