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. Author manuscript; available in PMC: 2020 Jul 18.
Published in final edited form as: Methods Enzymol. 2019 Jul 18;626:447–474. doi: 10.1016/bs.mie.2019.06.028

Figure 4.

Figure 4.

Cell lysate should max out the UV detector at approximately 3,000 mAu with a prominent protein peak following elution. This is the maltose binding protein-zCD2 fusion protein. A representative HisTrap™ HP column chromatogram is shown.