Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The zCD2 protein should no longer have a His6 tag and therefore should no longer bind to the HisTrap™ HP column. However, some zCD2 may bind nonspecifically, which is easily discernible by SDS-PAGE. A representative HisTrap™ HP column chromatogram from the second purification step and gel are shown.
