FIG 2.

YceF confers increased Mn(II) tolerance, but has little effect on sensitivity to other tested metals. yceF mutant sensitivity to Mn(II) (10 μl of 100 mM MnCl₂/1 M FeSO₄/100 mM ZnCl₂). PS indicates the mneP mneS background. Mid-logarithmic cells (OD₆₀₀, ∼0.4) were plated on LB agar plates and overlaid with 10 μl of each metal on a filter paper disk. The zone of growth inhibition was measured after overnight growth. Data represent the means ± the SD for at least three biological replicates. *, P < 0.05; ***, P < 0.001; ns, no statistical significance (as determined using an unpaired two-tailed Student t test).