FIG 5.

yceF and ykoY mutants in a mneP mneS background (PS) confer Mn(II) tolerance by reducing internal Mn(II) accumulation. ICP-MS analysis was used to measure intracellular Mn(II) accumulation after Mn(II) shock. Wild-type and mutant cells were grown to mid-logarithmic phase (OD₆₀₀, ∼0.4), 100 μM MnCl₂ was added to the LB medium, and samples were collected before shock (“0”) and 30 min after shock (“30”). Mn(II) levels were normalized against total protein. Data represent means for two biological replicates performed at different times, and error bars indicate the ranges. ***, P < 0.001; ****, P < 0.0001; ns, no statistical significance (as determined using an unpaired two-tailed Student t test).