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Substrate screening for PfuEndoQ. Various modified or damaged bases containing DNA (A) (see Table S1 in the supplemental material) were incubated in a reaction mixture with or without PfuEndoQ at 60°C for 10 min. DNA products were separated by 8 M urea-12% PAGE, and 32P-labeled DNA strands were detected by autoradiography. (A) Cleaved substrates by PfuEndoQ are highlighted in red. (B) dsDNA. (C) ssDNA. −, no enzyme; +, 100 nM PfuEndoQ; marker, 13 nt.
