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PfuEndoQ generates a nick at the 5′ sides of 5hU, 5hC, and DHU. (A) The nicking sites of PfuEndoQ in 5hU-, 5hC-, and DHU-containing DNA. (B and C) DNA substrates were incubated with or without PfuEndoQ WT or E76A (10 nM) at 60°C for 10 min in a reaction mixture. DNA products were separated by 8 M urea-12% PAGE (sequencing gel), and 32P-labeled DNA strands were detected by autoradiography. (B) dsDNA. (C) ssDNA. −, no enzyme; marker, 13 and 14 nt.
