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(A and B) Effect of pre-irradiation treatment with silymarin at different time intervals on the generation of ROS and changes in MMP, in RKO and HCT-15 cells. After various treatments, ROS levels were measured fluorimetrically, by using CM-DCFH2DA or DiOC 6(3) (Ex λ: 488 nm; Em λ: 530 nm; FL1 region) as described in materials and methods section. The mean fluorescence emission values are shown along with histograms.
