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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Hepatol. 2019 Oct 10;72(1):146–155. doi: 10.1016/j.jhep.2019.09.030

Figure 2. Impact of APAP-induced acute liver injury on VWF multimer distribution and VWF activity.

Figure 2.

Male wild-type mice were given 300mg/kg APAP or saline (i.p.) and samples were collected 24h, 48h, and 72h later. (A) Representative images of VWF multimer analysis from normal pooled mouse plasma (NPP) (first lane) and plasma from saline-treated (second lane) and APAP-challenged wild-type mice (third to fifth lane) using a semi-automated gel electrophoresis method. (B) Representative densitometry tracings of VWF multimers comparing normal pooled mouse plasma (grey line) to challenged mice (pink line). (C) The proportion of HMW VWF multimers (relative to total VWF) in plasma was quantified by software-assisted morphometric analysis. (D) VWF collagen binding activity (VWF:CB) in plasma samples from wild-type APAP-challenged mice, shown as a percentage compared to normal pooled mouse plasma, set to 100%. (E) Ratio of VWF:CB and VWF:Ag (VWF:CB/VWF:Ag) in plasma samples from APAP-challenged wild-type mice. LMW = Low molecular weight. HMW = High molecular weight. Bars represent mean + standard error of the mean (n=3 mice per group for panel C, n= 5-12 mice per group for panel D-E), * p <0.05 compared to saline-treated animals.