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. Author manuscript; available in PMC: 2020 Dec 11.
Published in final edited form as: Cell Host Microbe. 2019 Nov 19;26(6):715–728.e8. doi: 10.1016/j.chom.2019.10.003

Figure 2. Binding of N9-Reactive mAbs Is Subtype-Specific and Is not Affected by Mutations Causing Resistance to Neuraminidase Inhibitors.

Figure 2.

(A) A panel of 35 human mAbs was isolated on the basis of reactivity to recombinant SH13 N9 NA antigen. Cross-reactivity of N9-reactive mAbs to recombinant NA proteins from group 1 was measured by ELISA. Representative EC50 values (ng/mL) from two independent experiments are plotted as a heatmap. NAs were clustered by amino acid sequence phylogeny. Three mAbs that bind N9 antigen with EC50 value higher than 10 μg/mL (highest tested concentration) excluded from representation.

(B) Binding of N9-reactive mAbs to recombinant wild-type N9 NA from A/Shanghai/2/2013 virus or NI-resistant mutants was measured by ELISA. Representative EC50 values (ng/mL) from two independent experiments are plotted as a heatmap. Four mAbs that bind N9 SH13 antigen with EC50 value higher than 10 μg/mL (highest tested concentration) excluded from representation.

See also Table S4 and Figure S2.