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. Author manuscript; available in PMC: 2020 Dec 11.
Published in final edited form as: Cell Host Microbe. 2019 Nov 19;26(6):715–728.e8. doi: 10.1016/j.chom.2019.10.003

Figure 3. Anti-N9 Human mAbs Inhibit Egress of A/Shanghai/2/2013 IDCDC-RG32A H7N9 Virus, Mainly by Blocking the N9 Enzyme Active Site.

Figure 3.

Individual mAbs were assessed for H7N9 virus neutralization by using hemagglutination inhibition (HAI), egress inhibition, enzyme-linked lectin (ELLA) neuraminidase inhibition (NI) and NA-Fluor NI assays.

(A) Egress inhibition of H7N9 virus by mAbs. IC100 values (nM) are shown as mean ± SD of three technical replicates. Four mAbs with low expression were excluded from the analysis.

(B) Inhibition of N9 enzymatic activity by mAbs measured in ELLA. The assay used fetuin as substrate and H7N9 virus as the enzyme source. Data represent one of two independent experiments, shown as mean ± SD of three technical replicates.

(C) Inhibition of N9 enzymatic activity by mAbs measured in NA-Fluor assay. The assay used H7N9 virus as the enzyme source. Data are shown as mean ± SD of three technical replicates.

(D) mAbs functional activity comparison. Representative IC100 values (nM) from HAI or egress inhibition assays and IC50 values (nM) from ELLA NI or NA-Fluor NI assays are plotted as a heatmap. mAb NA-152 with low expression were excluded from the analysis.

See also Table S5.