Table 1.
Effects of VTA Drd2 mRNA knockdown on rates of local cerebral glucose utilization in rat brain
| Controls | Treated | ||
|---|---|---|---|
| Brain Region | PBS (N=5) | SCR (N=8) | Drd2KD (N=8) |
| Mesolimbic | |||
| Rostral accumbens | 89.0 ± 7.4 | 82.9 ± 4.0 | 101.6 ± 3.6 ** |
| Accumbens core | 79.0 ± 5.1 | 77.4 ± 3.5 | 97.9 ± 5.3 ** |
| Accumbens shell | 71.8 ± 4.9 | 69.8 ± 4.3 | 93.0 ± 7.6 ** |
| Olfactory tubercle | 77.4 ± 7.0 | 76.2 ± 5.5 | 96.6 ± 6.0 * |
| Ventral pallidum | 64.8 ± 4.0 | 57.7 ± 3.0 | 74.3 ± 4.1 * |
| Ventral tegmental area | 59.2 ± 3.8 | 54.7 ± 4.2 | 67.6 ± 2.8 * |
| Lateral habenula | 97.9 ± 6.6 | 97.8 ± 6.0 | 116.1 ± 5.3 * |
| Nigrostriatal | |||
| Dorsal caudate | 106.4 ± 7.1 | 100.3 ± 5.5 | 127.8 ± 6.2 ** |
| Lateral caudate | 111.4 ± 7.8 | 103.5 ± 6.1 | 129.7 ± 5.1 ** |
| Ventral caudate | 102.5 ± 5.1 | 99.1 ± 4.3 | 118.3 ± 4.3 ** |
| Globus pallidus | 63.2 ± 4.4 | 53.1 ± 4.3 | 67.0 ± 3.0 |
| Entopeduncular nucleus | 48.9 ± 3.3 | 47.7 ± 3.3 | 56.8 ± 2.1 ** |
| Subthalamic nucleus | 83.9 ± 5.3 | 86.3 ± 4.4 | 105.8 ± 3.2 ** |
| Substantia nigra compacta | 68.4 ± 3.7 | 63.2 ± 3.5 | 75.9 ± 2.7 * |
| Substantia nigra reticulata | 50.4 ± 3.6 | 51.2 ± 2.7 | 60.3 ± 2.9 ** |
| Limbic | |||
| Lateral septum | 61.9 ± 5.5 | 58.4 ± 3.3 | 74.3 ± 3.2 ** |
| Medial forebrain bundle | 58.9 ± 4.4 | 58.4 ± 4.1 | 68.7 ± 3.1 * |
| Basolateral amygdala | 72.0 ± 4.9 | 69.8 ± 3.7 | 84.6 ± 3.7 ** |
| Central amygdala | 40.9 ± 2.7 | 39.2 ± 2.2 | 49.5 ± 1.8 ** |
| Hippocampus CA1 | 63.3 ± 4.4 | 62.1 ± 3.5 | 76.2 ± 4.9 * |
| Hippocampus CA3 | 66.9 ± 4.6 | 69.0 ± 3.0 | 80.0 ± 3.5 * |
| Dentate gyrus | 54.6 ± 2.5 | 57.6 ± 3.0 | 68 9 ± 2.5 ** |
| Cortex and Thalamus | |||
| Medial prefrontal cortex | 71.9 ± 5.7 | 68.0 ± 3.5 | 86.5 ± 3.4 ** |
| Anterior cingulate | 110.2 ± 8.3 | 105.7 ± 5.9 | 131.5 ± 5.5 ** |
| Motor cortex | 101.4 ± 7.4 | 102.4 ± 5.7 | 127.1 ± 5.1 ** |
| Corpus callosum | 38.7 ± 4.3 | 37.0 ± 2.9 | 44.5 ± 2.6 |
| Lateral thalamus | 81.2 ± 6.7 | 87.6 ± 5.0 | 100.6 ± 4.3 * |
| Medial thalamus | 91.4 ± 5.0 | 92.2 ± 4.9 | 110.0 ± 4.4 ** |
| Habenula | 66.5 ± 4.3 | 69.4 ± 4.0 | 80.0 ± 3.3 * |
| Brainstem | |||
| Superior colliculus | 91.3 ± 5.7 | 85.3 ± 5.7 | 100.6 ± 4.0 |
| Dorsal raphe | 80.7 ± 6.5 | 80.4 ± 4.2 | 91.1 ± 6.0 |
| Median raphe | 89.4 ± 5.9 | 82.7 ± 5.8 | 100.0 ± 4.6 |
| Locus coeruleus | 59.1 ± 5.9 | 58.7 ± 4.7 | 71.7 ± 3.3 * |
| Cerebellum | 72.2 ± 6.4 | 70.5 ± 4.7 | 84.9 ± 3.7 * |
Data are expressed as mean (μmol/100 g/ min) ± S.E.M.
*
p <.025,
**
p <.010 compared to combined control groups