Fig. 2.

Lactobacillus plantarum TCI378 (TCI378) possessed the bile salt hydrolase activity and reduced the emulsification effect of bile salts. (A) TCI378 was streaked on MRS agar plates (MRS) or MRS agar plates containing 0.5% taurodeoxycholic acid (MRS+TDCA). Comparing to the regular TCI378 colonies (close view of left plate), TCI378 formed opaque granular white colonies on MRS+TDCA plates (close view of right plate). (B) The purified metabolite of TCI378 was added to the MRS medium containing bile salts and soy oil [TCI378(m)]. After mix the oil-water interface, the emulsification was observed. The emulsified layer was depleted in the mixture containing TCI378(m) after incubation at 37°C for 30 min, while the emulsification remained in the control (mock).