Fig. 4.

Lactobacillus plantarum TCI378 (TCI378) metabolites inhibit 3T3-L1 cell adipogenesis and lipolysis. Differentiated 3T3-L1 cells were left untreated (Mock), treated with 1% MRS (blank; MRS), or treated with 1% culture supernatant without TCI378 cells (TCI378), and cultured for 2 days. (A) Images of Oil red O-stained 3T3-L1 cells on plates. (B) Levels of oil accumulation were determined by quantifying Oil red O staining. (C) Real-time RT-PCR assays were performed for the adipogenic marker gene GLUT4 and lipolytic maker gene PLIN1. Data are presented as mean±SD. *P <0.05 and **P <0.01 compared with control, and ^#P <0.05 and ^###P <0.001 compared with MRS (n=3).