Fig. 4.

Effect of seed extract of Quisqualis indica (HU-033) on the expression of androgen receptor (AR) and estrogen receptor (ER) α in vivo. (A) Representative photomicrographs of prostate tissues (magnification × 400, left). The percentage of AR-positive area in the prostate tissue (right). (B) Representative photomicrographs of prostate tissues (magnification ×400, left). The percentage of ERα-positive area in the prostate tissue (right). (C) The protein expression of AR, ERα, and β-actin in rat prostate tissues was analyzed by using western blotting. (D) The relative ratio of band intensity was calculated for AR/β-actin and ERα/β-actin. ^#P <0.05, ^##P <0.01, and ^###P <0.001 vs. the normal control (NC) group and ^**P <0.01 ^***P <0.001 vs. the benign prostatic hyperplasia (BPH) group.