Figure 5 -. Activation of the Gq/11-linked dopamine receptor stimulates calcium release in human macrophages.
Human MDM were loaded with the fluorescent calcium indicator dye Oregon Green Bapta-488 for an hour, followed by treatment with the indicated drug. A represents calcium traces over the time period whereas B shows the area under the curve analysis. Dopamine (gray) significantly increased calcium release over the vehicle control (white line). Treatment with the Gq/11 inhibitor YM-254,890 partially attenuated this dopamine mediated increase (blue). YM alone (pink) had no effect on calcium flux compared to the vehicle control. Ionomycin (inset, orange line) was used as a positive control. All data was normalized to baseline, and the vehicle control was set to one. Each N represents an average of 75-110 cells from 3 - 5 individual donors. **p<0.01, **** p<0.0001