Figure 7 -. Inhibition of Gq/11 modulates PKC phosphorylation in macrophages.
MDM were pre-treated with the Gq/11 inhibitor YM-254890 for thirty minutes followed by treatment with vehicle or 10−6M DA for one minute (A, B). Cells were lysed and analyzed for phospho-PKC via Western Blot. A representative western blot is shown in (A, 1-Untx, 2-PMA 10−6 M, 3-DA 10−6 M, 4-YM-254890 10−6 M, 5-YM+DA). Analysis using a 2way ANOVA showed a significant interaction between dopamine and YM-254890, and post hoc analyses showed that dopamine alone (B, gray columns) significantly increased PKC phosphorylation over the untreated vehicle (white columns), while this effect was not present in MDM treated with YM-254,890. *p<0.05 Data are represented as fold change compared to the mean of the vehicle treated control.