Fig. 2.

Effects of Tat-CIAPIN1 protein on LPS-induced inflammatory responses in Raw 264.7 cells. The cells were treated with Tat-CIAPIN1 (3 μM) or CIAPIN1 protein for 1 h before being exposed to LPS (1 μg/ml). MAPK and NF-κB activation (A) and the expression levels of COX-2 (B) and iNOS (C) protein were analyzed by Western blotting. Total RNA was extracted from the cells. We analyzed cytokines (IL-6 and TNF-α) and GAPDH mRNA by RT-PCR using specific indicated primers (D). The band intensity was measured by densitometer. *P < 0.05, compared with LPS-treated cells.