Table 2. Hepacivirus protein length and cleave site sequences.

Protein	Core		E1		E2		P7		NS2		NS3		NS4A		NS4B		NS5A		NS5B
Virus	aa	cleavage	aa	cleavage	aa	cleavage	aa	cleavage	aa	cleavage	aa	cleavage	aa	cleavage	aa	cleavage	aa	cleavage	aa
A/Equine hepacivirus/JPN3/Ec	204	GEA$|$SV	188	VSC$|$TD	335	AEA$|$YL	63	AWA$|$FD	217	RLL$|$SP	631	TQT$|$NA	54	EEC$|$FD	257	QNC$|$DF	406	ESC$|$SL	588
B/Hepatitis GB virus B	156	CSG$|$AR	193	TSG$|$NP	264	AGL$|$PL	119*	ASA$|$FD	208	AIT$|$AP	620	VNT$|$SG	55	EEC$|$AS	248	DDC$|$GL	411	FSC$|$SM	590
C/Hepatitis C virus genotype 1	191	ASA$|$YQ	192	VDA$|$ET	363	AEA$|$AL	63	AYA$|$LD	217	RLL$|$AP	631	VVT$|$ST	54	EEC$|$SQ	261	TPC$|$SG	448	VCC$|$SM	591
D/Guereza hepacivirus/GHV-2/Cg	181	GAS$|$CV	211	VTS$|$TS	260	AAA$|$AA	67	AVG$|$FD	208	SML$|$NP	625	NDC$|$SL	60	EEC$|$SF	248	AQC$|$DG	882	AKC$|$AS	591
E/Rodent hepacivirus/RHV-339/Pm	168	ATA$|$VS	185	AAA$|$AA	280	AYA$|$FT	54	AYA$|$AS	198	KYT$|$IP	621	FFA$|$SG	58	EEC$|$YN	246	DLC$|$TP	355	HSC$|$SM	583
F/Hepacivirus F/NLR07/Mg	149	AVT$|$NC	184	AAA$|$AS	280	AFA$|$FT	54	TSA$|$YS	199	ERT$|$AP	621	YFA$|$ST	58	EEC$|$YQ	246	EDC$|$SC	479	HEC$|$SS	585
G/Norway rat hepacivirus 1/NYC-C12/Rn	208	ASA$|$GI	243	VAA$|$PV	271	VGA$|$LE	55	EAY$|$EG	198	RFT$|$AP	621	YFA$|$ET	57	EEC$|$ST	249	DVC$|$TS	506	TDC$|$SW	583
H/Norway rat hepacivirus 2/NYC-E43/Rn	165	AEA$|$NL	216	SAV$|$AV	272	SEA$|$VP	56	RAE$|$QF	197	QLT$|$KP	620	YYC$|$GL	61	EEC$|$AN	239	EIC$|$DG	449	SSC$|$SK	580
I/Hepacivirus/SAR-3/Rp	172	VEP$|$KP	197	SVA$|$AP	255	YAQ$|$PP	53	VEA$|$FS	204	QLS$|$SP	622	ELA$|$SW	56	EEC$|$AL	251	EPC$|$TD	385	ETC$|$TY	586
J/Hepacivirus J/RMU-3382/Mg	163	AVS$|$HW	185	AEG$|$LP	287	ANA$|$LV	44	AQG$|$GC	236	RLT$|$AP	625	EEM$|$TD	58	EEC$|$GF	258	AEC$|$AG	555	TSC$|$NY	603
K/Hepacivirus K/PDB-829/Hv	190	GEA$|$SY	193	AQA$|$NP	325	ADA$|$AL	63	AVG$|$GP	217	RHC$|$SP	628	DDT$|$ST	56	EEC$|$LS	257	SEC$|$AF	504	DEC$|$SA	591
L/Hepacivirus L/PDB-112/Hv	161	AES$|$VP	203	AAA$|$MP	265	AWG$|$WP	59	AQA$|$AS	212	ERN$|$AP	629	YSA$|$GG	57	EEC$|$MQ	259	AEC$|$DG	451	ESC$|$SE	605
M/Hepacivirus M/PDB-491.1/Om	189	VDA$|$SF	193	SQA$|$AE	321	ALA$|$VP	63	VDA$|$YT	217	RHC$|$SP	628	TPT$|$SA	55	EEC$|$AD	259	RNC$|$SC	456	SPC$|$SA	590
N/Bovine hepacivirus/GHC25/Bt	155	VSG$|$YR	190	VEA$|$TT	267	ATA$|$AL	60	VTA$|$LD	204	APC$|$AP	624	LDV$|$WG	54	EEC$|$WG	250	VPC$|$GF	390	KEC$|$SY	579
Hepacivirus P/RHV-GS2015/Cd	153	GLA$|$FT	186	VAA$|$PV	270	AEG$|$AM	56	VLG$|$AS	199	KRT$|$AP	622	YFA$|$ST	58	EEC$|$SL	245	DFC$|$SP	395	SDC$|$SY	580
Sifaka hepacivirus	162	VGA$|$AF	194	AAA$|$AP	269	VEA$|$VP	55	VEA$|$YT	198	RLT$|$AP	622	FFT$|$AW	58	EEC$|$AL	236	TLC$|$AS	386	QAL$|$SQ	576
Wenling shark virus	300	MDS$|$AP	194	AVA$|$AP	214	AEA$|$SV	71	ALG$|$DD	224	NRC$|$AP	632	LVA$|$GL	56	DLS$|$GA	314	VVM$|$AD	475	SPM$|$SH	606
Duck hepacivirus strain HCL-1/Ap	290	ASA$|$DH	195	GMA$|$DR	274	AEG$|$ML	74	VLG$|$AS	245	QYT$|$AP	625	NCS$|$AA	55	EEC$|$SA	250	YEC$|$NS	1006	ESC$|$SF	593
Jogalong virus	87	AVA$|$FS	191	AQA$|$GT	306	IEG$|$AV	74	VAG$|$ED	235	KLA$|$AP	625	SAG$|$LT	52	EEC$|$AS	247	TNC$|$TS	536	VCC$|$GE	588

*p13 is processed into p6 (57aa) and p7 (62aa) in GBV-B; $|$, location of predicted proteolytic cleavage; further information for each virus can be found in S1 Table.