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. 2019 Dec 9;8:e53535. doi: 10.7554/eLife.53535

Table 2. Kinetic parameters for GSA and NAGSA dehydrogenase activities of ProA, ProA*, and ProA**.

	GSA activity (ProA)			NAGSA activity (neo-ArgC)
	k_cat (s⁻¹)	K_M (mM)	k_cat/K_M,GSA (M⁻¹ s⁻¹)	k_cat (s⁻¹)	K_M (mM)	k_cat/K_{M, NAGSA} (M⁻¹ s⁻¹)
WT	16 ± 0.3	0.22 ± 0.01	72000 ± 2000	0.0083 ± 0.0009	0.30 ± 0.09	28 ± 9
ProA* (E383A)	0.0076 ± 0.0008	0.20 ± 0.04	37 ± 8	0.046 ± 0.002	0.076 ± 0.009	610 ± 74
ProA** (E383A F372L)	0.023 ± 0.005	0.42 ± 0.14	55 ± 22	0.21 ± 0.01	0.095 ± 0.011	2200 ± 260

^a Values reported were calculated from a nonlinear least squares regression of three replicates at each substrate concentration ± standard error.