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. Author manuscript; available in PMC: 2020 Jan 3.
Published in final edited form as: Methods Mol Biol. 2009;485:163–184. doi: 10.1007/978-1-59745-170-3_12

Fig. 12.4.

Fig. 12.4.

Mutations in Gag lead to defects in virus release. HeLa cells transfected with WT pNL4-3 or the indicated Gag mutants that are defective in virus release or Env expression were metabolically labeled with [35S]Met/Cys, and virions were pelleted by ultracentrifugation. Labeled viral proteins in cell and virion lysates were immunoprecipitated with HIV-Ig and analyzed by SDS-PAGE. Mutant clones are as follows: 1GA, nonmyristylated Gag mutant; WM184,185AA, CA C-terminal domain dimer interface mutant; 15A, clone in which all basic residues in NC were mutated to Ala; PTAP−, mutant bearing substitutions in all four PTAP late domain residues; KFS, Env-minus molecular clone.