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. 2020 Jan 3;11:91. doi: 10.1038/s41467-019-13765-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Reactions were carried out as described in the Experimental section. All samples were tested in three biological replicates. Image of representative data is shown here. Uncleaved t-GFP1 DNA (702 bp) cut to shorter cleavage fragments (469 bp and 233 bp) were indicated. a, b The influence of chemical masking of gRNA on Cas9 cleavage of t-GFP1. The gRNA (gRNA-GFP) was synthesized by in vitro transcription with T7 RNA polymerase. c, d The influence of DPBM on Cas9 cleavage of t-GFP1. The CRISPR/Cas9 system with acylated gRNA-GFP (200 mM NAI-N₃, 2 h) was treated with various concentrations of DPBM. Source data is available in the Source Data file.