Fig. 2. Candidate AIS proteins and their sensitivity to detergent extraction.

a The ratio (Log₂) of PSMs in NF186-BirA*-expressing neurons to PSMs in NF186∆FIGQY-BirA*-expressing neurons. Known AIS proteins are shown in red. An endogenously biotinylated carboxylase (Acaca), that is equally biotinylated in both NF186-BirA* expressing neurons and NF186∆FIGQY-BirA* expressing neurons, is shown for reference. Dashed lines at 1 indicate twofold enrichment, or equal levels of biotinylation at 0. n = 4 independent experiments. Error bars, ±SEM. b Immunostaining of DIV14 hippocampal neurons using antibodies against AnkG (red), α2 spectrin (green), and Map2 (blue). The lower panels show the Triton X-100 detergent-resistant pool of α2 spectrin at the AIS. AIS are indicated by arrowheads. Scale bar = 5 μm. c Immunostaining of DIV14 hippocampal neurons using antibodies against AnkG (red), Arhgap21 (green) and Map2 (blue). The lower panels show Arhgap21 is solubilized by Triton X-100. AIS are indicated by arrowheads. Scale bar = 10 μm.