Table 1.
# Parasites (1n) | # Parasites (>1n) | |||
---|---|---|---|---|
Day p.i. | GFP +ve only | GFP and mCherry +ve | GFP +ve only | GFP and mCherry +ve |
2 | 170 | 0 | 0 | 0 |
3 | 143 | 1 | 0 | 0 |
4 | 71 | 9 | 0 | 24 |
6 | 58 | 1 | 0 | 37 |
10 | 36 | 3 | 0 | 19 |
Numbers of parasites in one well (96-well plate) expressing GFP only, or expressing both GFP and mCherry. Parasites were scored after fixation at different time points p.i. (post infection). Parasites were categorized as 1n (containing 1 nucleus) or >1n (multiple nuclei) as determined by the DAPI signal