Fig. 1. ROCK inhibitors increase Parkin recruitment to damaged mitochondria.

a Average activity (% of cells with mitochondrial Parkin distribution) of all compounds screened. Based on these values, compounds are classified as either activators (green), samples (yellow) or inhibitors (pink). ROCK inhibitors Y27632 (light blue), Y39983 (navy) and SR3677 (light green) are highlighted. b Enhancers of Parkin recruitment from a arranged according to common protein target. ROCK inhibitors Fasudil (yellow), rho kinase inhibitor II (olive) and GSK2699662A (green). Pink dashes represent average value amongst each group and pink dots represent activity values for activator compounds whose canonical target is not ROCK. c GFP Parkin distribution in cells following incubation in 4 µM of small-molecule library compound for 16 h and mitophagy induction with 2 h of 20 µM CCCP treatment (n = 2 independent experiments). Scale bars, 10 µm. d HEK293 GFP Parkin cells were treated with varying doses of SR3677 for 2 h prior to induction of mitophagy with 10 µM CCCP for 1 h. Dose response curves were fitted to these data, yielding an EC₅₀ value of 57 nM. Data are expressed as mean ± s.e.m (n = 3 independent experiments). Error bars represent s.e.m. e Chemical structures of ROCK inhibitors identified.