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. 2020 Jan 3;6:2. doi: 10.1038/s41523-019-0143-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic diagram of MELK promoter cloned into PGL2-basic luciferase reporter plasmid with predicted p53-binding elements within −5.0 kb. b Luciferase assay to determine MELK promoter activity in MCF7 cells after knocking down of wild-type p53 using shRNA. Inset shows the knockdown of p53. c Luciferase assay to determine MELK promoter activity in p53-null cells (MDA 436) after inducing wild-type p53 expression using doxycycline-inducible system. Inset shows the induction of p53 protein by doxycycline (Dox) treatment. d Luciferase assay to determine MELK promoter activity in p53-null cells (SKOV3) after inducing wild-type p53 expression using doxycycline-inducible system. Inset shows the induction of p53 protein by doxycycline (Dox) treatment. e Schematic diagram of MELK promoter constructs. f Luciferase assay to determine MELK promoter (shown in e) activity in MDA 436 cells after inducing wild-type p53. g Schematic diagram of MELK promoter constructs. h Luciferase assay to determine MELK promoter (shown in g) activity in MDA 436 cells after inducing wild-type p53. For luciferase assays a minimum of three replicates were used. *Statistical significance of p-value < 0.05. Error bars represent ± SD.