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. 2020 Jan 3;5:1. doi: 10.1038/s41541-019-0151-3

Fig. 1. Humoral responses induced by iFMDV, Ad5-FMDV, and Ad5-FMDV+ISA206VG vaccine in sheep.

Fig. 1

a Experimental design: Three groups of 10 sheep each were immunized with iFDMV (intramuscular route), or Ad5-FMDV with or without ISA206VG (half intramuscular and half subcutaneous route). Blood was collected on PAXgene® Blood RNA tubes at T0H, T4H, and T24H and processed to RNA-seq. Sera were collected at T0H and every 2 weeks for 365 days. b The mean percent inhibition values provided by the PrioCHECK ELISA in each group at each time point are plotted over time (365 days), and the standard errors of the mean are shown above the mean (10 sheep per group). Differences between the areas under the curve over time of the three groups are statistically significant (p < 0.001, Wilcoxon test). The doubtful and positivity thresholds (42% and 58% respectively) are shown with a dashed line. c The mean VNT titer per group are plotted over time and the s.e.m. are shown above the mean. Differences between the areas under the curve of the iFMDV group and the other groups are statistically significant (p < 0.005, Wilcoxon test). The differences between the areas under the curve of the Ad5-FMDV and Ad5-FMDV+ISA206VG groups are not significant. The doubtful and positivity thresholds (VNT titers of 32 and 45 respectively) are shown with a dashed line. d The mean percent inhibition values provided by the SPCE test in each group at each time point are plotted over time (365 days), and the s.e.m. are shown above the mean. Differences between the areas under the curve over time of the three groups are statistically significant (p < 0.005, Wilcoxon test). The doubtful and positivity thresholds (40% and 60% respectively) are shown with a dashed line.