Fig. 8. Transcriptomic signatures of the Ab response magnitude induced by the Ad5-FMDV+ISA206VG vaccine.

A PLS algorithm was used to retrieve the genes with fold changes (T24H vs. T0H) related to the PrioCHECK area under the curve. The ranked gene list of the first PLS component was processed through a GSEA to identify enriched BTMs (nominal p value of the GSEA < 0.05, p < 0.25). The gene fold changes contributing to each BTM were computed into a single activity score and a Spearman correlation test to VNT was performed across the 10 sheep (p < 0.05). a The BTMs positively correlating with the Ab response magnitude are in graded red according to the Spearman rho coefficient, and the negatively correlating ones are in graded blue. The BTMs are grouped in functional families (T cell (1), B cell (2), neutrophils (3)). b The fold changes of genes contributing to the selected BTM (y-axis) are represented in a violin plot for each sheep which are ordered (x-axis) according to their PrioCHECK area under the curve values (720, 772, 021, 757, 662, 673, 743, 796, 751, 024). Each sheep was attributed a color, red in the maximal responders, yellow in intermediate responders, and blue in the lower responders (see Fig. 4). Four modulated BTMs, selected on their biological meaning and top positions, are illustrated. The list of the enriched BMTs and their statistical value is provided in Supplementary Data Set 7 and the list of the genes contributing to the selected BMTs and their fold changes in high and low responders is shown in Supplementary Fig. 5.