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. 2019 Nov 19;146(1):53–66. doi: 10.1007/s00432-019-03082-z

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Digital image analysis: Digital image analysis was used to quantify the spatial distribution of tumor-associated neutrophils (TANs) in gastric cancer. The viewer and painting program VMP was used to mark the tumor compartments: tumor surface (green), tumor center (yellow) and invasion front (orange; a, b). The TAN density was lower in the tumor center (c) than in the invasion front (d–f). TAN density was quantified by image analysis using Definiens Tissue Studio^® (TANs identified by Definiens are marked as yellow dots; f). All figures were captured from the same tumor. Anti-myeloperoxidase immunostaining (a, b, e, f). Hematoxylin and eosin (c, d). Overview (a, b). Original magnification 400-fold (c–f)