Table 2.
Correlation of the densities of myeloperoxidase-immunoreactive tumor-associated neutrophils dichotomized into quartile 1 vs. quartile 2–4 with clinicopathological patient characteristics. (1) Fisher’s exact test. (2) Kendall’s tau test. (3) Log-rank test
| Total | Mucosa | Tumor surface | Tumor center | Invasion front | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q1 | Q234 | Q1 | Q234 | Q1 | Q234 | Q1 | Q234 | |||||||||||||
| Valid/missing value | Valid | Valid | Valid | Valid | ||||||||||||||||
| n/n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | n | (%) |
n n |
(%) |
n n |
(%) | n | (%) | |||
| Gender | n | p value(1) | 449/0 | 257 | 0.242 | 358 | 0.899 | 449 | 0.071 | 386 | 0.013 | |||||||||
| Female | 164 | (36.5) | 30 | (28.8) | 74 | (71.2) | 31 | (24.2) | 97 | (75.8) | 49 | (29.9) | 115 | (70.1) | 43 | (32.8) | 88 | (67.2) | ||
| Male | 285 | (63.5) | 34 | (22.2) | 119 | (77.8) | 58 | (25.2) | 172 | (74.8) | 63 | (22.1) | 222 | (77.9) | 53 | (20.8) | 202 | (79.2) | ||
| Age group | n | p value(1) | 449/0 | 257 | 0.774 | 358 | 0.142 | 449 | 0.744 | 386 | 0.906 | |||||||||
| < 68 years | 221 | (49.2) | 32 | (24.1) | 101 | (75.9) | 49 | (28.7) | 122 | (71.3) | 57 | (25.8) | 164 | (74.2) | 46 | (24.5) | 142 | (75.5) | ||
| ≥ 68 years | 228 | (50.8) | 32 | (25.8) | 92 | (74.2) | 40 | (21.4) | 147 | (78.6) | 55 | (24.1) | 173 | (75.9) | 50 | (25.3) | 148 | (74.7) | ||
| Localisation | n | p value(1) | 440/9 | 250 | 1.0 | 350 | 0.794 | 440 | 0.639 | 378 | 0.063 | |||||||||
| Proximal stomach | 143 | (31.8) | 20 | (26.0) | 57 | (74.0) | 30 | (26.1) | 85 | (73.9) | 38 | (26.6) | 105 | (73.4) | 25 | (19.5) | 103 | (80.5) | ||
| Distal stomach | 297 | (66.1) | 44 | (25.4) | 129 | (74.6) | 58 | (24.7) | 177 | (75.3) | 72 | (24.2) | 225 | (75.8) | 71 | (28.4) | 179 | (71.6) | ||
| Laurén type | n | p value(1) | 449/0 | 257 | 0.516 | 358 | 0.493 | 449 | <0.001* | 386 | <0.001* | |||||||||
| Intestinal | 239 | (53.2) | 29 | (22.7) | 99 | (77.3) | 50 | (25.0) | 150 | (75.0) | 42 | (17.6) | 197 | (82.4) | 34 | (15.3) | 188 | (84.7) | ||
| Diffuse | 136 | (30.3) | 24 | (27.9) | 62 | (72.1) | 28 | (28.0) | 72 | (72.0) | 54 | (39.7) | 82 | (60.3) | 51 | (54.3) | 43 | (45.7) | ||
| Mixed | 29 | (6.5) | 6 | (35.3) | 11 | (64.7) | 3 | (12.5) | 21 | (87.5) | 7 | (24.1) | 22 | (75.9) | 7 | (26.9) | 19 | (73.1) | ||
| Unclassified | 45 | (10.0) | 5 | (19.2) | 21 | (80.8) | 8 | (23.5) | 26 | (76.5) | 9 | (20.0) | 36 | (80.0) | 4 | (9.1) | 40 | (90.9) | ||
| T-category | n | p value(2) | 449/0 | 257 | 0.127 | 358 | 0.082 | 449 | 0.063 | 386 | 0.017 | |||||||||
| T1a/b | 52 | (11.6) | 7 | (17.9) | 32 | (82.1) | 9 | (20.5) | 35 | (79.5) | 7 | (13.5) | 45 | (86.5) | 8 | (15.7) | 43 | (84.3) | ||
| T2 | 53 | (11.8) | 10 | (27.8) | 26 | (72.2) | 10 | (20.8) | 38 | (79.2) | 12 | (22.6) | 41 | (77.4) | 8 | (15.7) | 43 | (84.3) | ||
| T3 | 185 | (41.2) | 21 | (20.8) | 80 | (79.2) | 34 | (22.8) | 115 | (77.2) | 48 | (25.9) | 137 | (74.1) | 43 | (26.7) | 118 | (73.3) | ||
| T4a/b | 159 | (35.4) | 26 | (32.1) | 55 | (67.9) | 36 | (30.8) | 81 | (69.2) | 45 | (28.3) | 114 | (71.7) | 37 | (30.1) | 86 | (69.9) | ||
| N-category | n | p value(2) | 449/0 | 257 | 0.011 | 358 | 0.468 | 449 | 0.174 | 386 | 0.751 | |||||||||
| N0 | 126 | (28.1) | 16 | (19.5) | 66 | (80.5) | 23 | (22.1) | 81 | (77.9) | 26 | (20.6) | 100 | (79.4) | 25 | (21.2) | 93 | (78.8) | ||
| N1 | 67 | (14.9) | 9 | (20.0) | 36 | (80.0) | 16 | (29.6) | 38 | (70.4) | 18 | (26.9) | 49 | (73.1) | 16 | (29.6) | 38 | (70.4) | ||
| N2 | 78 | (17.4) | 7 | (16.3) | 36 | (83.7) | 11 | (19.3) | 46 | (80.7) | 18 | (23.1) | 60 | (76.9) | 20 | (30.3) | 46 | (69.7) | ||
| N3a/b | 178 | (39.6) | 32 | (36.8) | 55 | (63.2) | 39 | (27.3) | 104 | (72.7) | 50 | (28.1) | 128 | (71.9) | 35 | (23.6) | 113 | (76.4) | ||
| M-category | n | p value(1) | 449/0 | 257 | 0.575 | 358 | 0.871 | 449 | 0.486 | 386 | 0.202 | |||||||||
| M0 | 364 | (81.1) | 51 | (24.2) | 160 | (75.8) | 73 | (24.6) | 224 | (75.4) | 88 | (24.2) | 276 | (75.8) | 76 | (23.5) | 247 | (76.5) | ||
| M1 | 85 | (18.9) | 13 | (28.3) | 33 | (71.7) | 16 | (26.2) | 45 | (73.8) | 24 | (28.2) | 61 | (71.8) | 20 | (31.7) | 43 | (68.3) | ||
| UICC stage 8th eds. | n | p value(2) | 449/0 | 257 | 0.195 | 358 | 0.482 | 449 | 0.118 | 386 | 0.115 | |||||||||
| IA/B | 74 | (16.5) | 11 | (20.8) | 42 | (79.2) | 13 | (20.3) | 51 | (79.7) | 12 | (16.2) | 62 | (83.8) | 13 | (18.1) | 59 | (81.9) | ||
| IIA/B | 101 | (22.5) | 13 | (20.3) | 51 | (79.7) | 21 | (25.6) | 61 | (74.4) | 26 | (25.7) | 75 | (74.3) | 23 | (25.6) | 67 | (74.4) | ||
| IIIA/B/C | 189 | (42.1) | 27 | (28.7) | 67 | (71.3) | 39 | (25.8) | 112 | (74.2) | 50 | (26.5) | 139 | (73.5) | 40 | (24.8) | 121 | (75.2) | ||
| IV | 85 | (18.9) | 13 | (28.3) | 33 | (71.7) | 16 | (26.2) | 45 | (73.8) | 24 | (28.2) | 61 | (71.8) | 20 | (31.7) | 43 | (68.3) | ||
| Total | Mucosa | Tumor surface | Tumor center | Invasion front | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q1 | Q234 | Q1 | Q234 | Q1 | Q234 | Q1 | Q234 | |||||||||||||
| Valid/missing value | Valid | Valid | Valid | Valid | ||||||||||||||||
| n/n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | |||
| Lymph node ratio | n | p value(1) | 449/0 | 257 | 0.006 | 358 | 0.328 | 449 | 0.326 | 386 | 0.906 | |||||||||
| < 0.189 | 220 | (49.0) | 26 | (18.2) | 117 | (81.8) | 40 | (22.3) | 139 | (77.7) | 50 | (22.7) | 170 | (77.3) | 49 | (25.3) | 145 | (74.7) | ||
| ≥ 0.189 | 229 | (51.0) | 38 | (33.3) | 76 | (66.7) | 49 | (27.4) | 130 | (72.6) | 62 | (27.1) | 167 | (72.9) | 47 | (24.5) | 145 | (75.5) | ||
| L-category | n | p value(1) | 423/26 | 242 | 0.106 | 338 | 0.528 | 423 | 0.824 | 365 | 0.904 | |||||||||
| L0 | 202 | (45.0) | 26 | (20.8) | 99 | (79.2) | 37 | (22.8) | 125 | (77.2) | 50 | (24.8) | 152 | (75.2) | 44 | (24.9) | 133 | (75.1) | ||
| L1 | 221 | (49.2) | 35 | (29.9) | 82 | (70.1) | 46 | (26.1) | 130 | (73.9) | 57 | (25.8) | 164 | (74.2) | 48 | (25.5) | 140 | (74.5) | ||
| V-category | n | p value(1) | 422/27 | 241 | 0.146 | 336 | 0.409 | 422 | 0.860 | 365 | 1.0 | |||||||||
| V0 | 374 | (83.3) | 58 | (26.7) | 159 | (73.3) | 70 | (23.3) | 230 | (76.7) | 95 | (25.4) | 279 | (74.6) | 82 | (25.4) | 241 | (74.6) | ||
| V1 | 48 | (10.7) | 3 | (12.5) | 21 | (87.5) | 11 | (30.6) | 25 | (69.4) | 11 | (22.9) | 37 | (77.1) | 11 | (26.2) | 31 | (73.8) | ||
| Tumor grade | n | p value(1) | 447/2 | 256 | 0.395 | 358 | 0.577 | 447 | 0.001* | 384 | 0.008 | |||||||||
| G1/2 | 108 | (24.1) | 12 | (19.7) | 49 | (80.3) | 25 | (27.2) | 67 | (72.8) | 14 | (13.0) | 94 | (87.0) | 16 | (15.2) | 89 | (84.8) | ||
| G3/G4 | 339 | (75.5) | 51 | (26.2) | 144 | (73.8) | 64 | (24.1) | 202 | (75.9) | 96 | (28.3) | 243 | (71.7) | 79 | (28.3) | 200 | (71.7) | ||
| Resection status | n | p value(1) | 436/13 | 248 | 0.109 | 348 | 0.694 | 436 | 0.503 | 374 | 0.717 | |||||||||
| R0 | 382 | (85.1) | 51 | (23.3) | 168 | (76.7) | 78 | (25.2) | 231 | (74.8) | 96 | (25.1) | 286 | (74.9) | 83 | (25.2) | 247 | (74.8) | ||
| R1/R2 | 54 | (12.0) | 11 | (37.9) | 18 | (62.1) | 8 | (20.5) | 31 | (79.5) | 11 | (20.4) | 43 | (79.6) | 12 | (27.3) | 32 | (72.7) | ||
| H. pylori status | n | p value(1) | 379/70 | 231 | 0.548 | 302 | 0.723 | 379 | 0.106 | 332 | 0.612 | |||||||||
| Negative | 320 | (71.3) | 50 | (26.2) | 141 | (73.8) | 57 | (23.0) | 191 | (77.0) | 88 | (27.5) | 232 | (72.5) | 71 | (25.6) | 206 | (74.4) | ||
| Positive | 59 | (13.1) | 8 | (20.0) | 32 | (80.0) | 14 | (25.9) | 40 | (74.1) | 10 | (16.9) | 49 | (83.1) | 12 | (21.8) | 43 | (78.2) | ||
| EBV status | n | p value(1) | 438/11 | 250 | 0.368 | 351 | 0.397 | 438 | 0.022 | 375 | 0.009 | |||||||||
| Negative | 416 | (92.7) | 61 | (26.0) | 174 | (74.0) | 79 | (23.7) | 254 | (76.3) | 108 | (26.0) | 308 | (74.0) | 93 | (26.1) | 264 | (73.9) | ||
| Positive | 22 | (4.9) | 2 | (13.3) | 13 | (86.7) | 6 | (33.3) | 12 | (66.7) | 1 | (4.5) | 21 | (95.5) | 0 | (0.0) | 18 | (100.0) | ||
| MSI status | n | p-value(1) | 437/12 | 249 | 0.009 | 352 | 0.813 | 437 | 0.035 | 375 | 0.002* | |||||||||
| MSS | 405 | (90.2) | 63 | (27.3) | 168 | (72.7) | 79 | (24.2) | 247 | (75.8) | 105 | (25.9) | 300 | (74.1) | 92 | (26.7) | 252 | (73.3) | ||
| MSI | 32 | (7.1) | 0 | (0.0) | 18 | (100.0) | 7 | (26.9) | 19 | (73.1) | 3 | (9.4) | 29 | (90.6) | 1 | (3.2) | 30 | (96.8) | ||
| HER2 status | n | p value(1) | 412/37 | 232 | 0.788 | 329 | 0.082 | 412 | 0.066 | 352 | 0.006 | |||||||||
| Negative | 376 | (83.7) | 53 | (25.0) | 159 | (75.0) | 68 | (22.9) | 229 | (77.1) | 98 | (26.1) | 278 | (73.9) | 87 | (27.4) | 231 | (72.6) | ||
| Positive | 36 | (8.0) | 4 | (20.0) | 16 | (80.0) | 12 | (37.5) | 20 | (62.5) | 4 | (11.1) | 32 | (88.9) | 2 | (5.9) | 32 | (94.1) | ||
| MET status | n | p value(1) | 440/9 | 253 | 0.408 | 351 | 0.808 | 440 | 0.665 | 378 | 1.0 | |||||||||
| Negative | 409 | (91.1) | 58 | (24.7) | 177 | (75.3) | 82 | (25.1) | 245 | (74.9) | 102 | (24.9) | 307 | (75.1) | 86 | (24.3) | 268 | (75.7) | ||
| Positive | 31 | (6.9) | 6 | (33.3) | 12 | (66.7) | 5 | (20.8) | 19 | (79.2) | 6 | (19.4) | 25 | (80.6) | 6 | (25.0) | 18 | (75.0) | ||
| OSS [months] | p value(3) | 0.052 | 0.134 | 0.142 | 0.017 | |||||||||||||||
| Total/Events/Censored | 433/341/92 | 60/50/10 | 186/139/47 | 88/70/18 | 258/204/54 | 109/88/21 | 324/253/71 | 92/75/17 | 278/207/71 | |||||||||||
| Median Survival | 15.0 ± 1.2 | 14.0 ± 2.6 | 18.8 ± 2.3 | 13.2 ± 2.1 | 16.7 ± 1.4 | 12.9 ± 1.6 | 16.0 ± 1.4 | 13.6 ± 2.2 | 16.7 ± 1.9 | |||||||||||
| 95% C.I. | 12.7–17.3 | 8.9–19.1 | 14.2–23.3 | 9.1–17.4 | 13.9–19.5 | 9.7–16.2 | 13.2–18.8 | 9.3–17.9 | 13.0–20.4 | |||||||||||
| TSS [months] | p value(3) | 0.073 | 0.156 | 0.137 | 0.013 | |||||||||||||||
| Total/Events/Censored | 405/280/125 | 49/35/14 | 179/111/68 | 84/60/24 | 238/161/77 | 103/75/28 | 302/205/97 | 87/65/22 | 260/164/96 | |||||||||||
| Median Survival | 16.7 ± 1.5 | 13.2 ± 3.8 | 22.6 ± 2.5 | 14.6 ± 3.5 | 18.0 ± 2.2 | 14.2 ± 2.0 | 17.9 ± 2.6 | 14.7 ± 2.9 | 19.6 ± 3.8 | |||||||||||
| 95% C.I. | 13.7–19.7 | 5.7–20.7 | 17.6–27.6 | 7.8–21.4 | 13.6–22.3 | 10.2–18.1 | 12.8–23.1 | 9.0–20.4 | 12.2–27.0 | |||||||||||
(1) Fisher’s exact test, (2) Kendall’s tau test, (3) Log-rank test, * Significant after FDR-Control