Table 2.
Precision oncotherapy tests of the 43 patients submitted for HPP, tailored chemotherapy and post-HPP targeted-therapy
| Pt | IV-CTCs (/ml) | 5-FU | Oxaliplatin | Irinotecan | Mitomycin | Doxorubicin | Cisplatin | Alkeran | Carboplatin | Raltitrexed | TC |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Part A | |||||||||||
| 1 | 14.8 | S = 70% | S = 75% | S = 70% | S = 82% | S = 19% | S = 70% | S = 25% | S = 70% | S = 60% | MMC (25 mg/m2) |
| 2 | 8.9 | S = 65% | S = 70% | S = 82% | S = 80% | S = 24% | S = 65% | S = 24% | S = 80% | S = 70% |
MMC (25 mg/m2) IRI (100 mg/m2) |
| 3 | 15.3 | S = 65% | S = 70% | S = 60% | S = 80% | S = 22% | S = 65% | S = 23% | S = 60% | S = 45% |
MMC (25 mg/m2) OX (80 mg/m2) |
| 4 | 12.2 | S = 48% | S = 61% | S = 48% | S = 82% | S = 23% | S = 70% | S = 23% | S = 47% | S = 28% | MMC (25 mg/m2) |
| 5 | 16.2 | S = 70% | S = 72% | S = 42% | S = 45% | S = 20% | S = 62% | S = 56% | S = 57% | S = 60% |
5-FU (1000 mg/m2) OX (80 mg/m2) |
| 6 | 7.5 | S = 62% | S = 75% | S = 80% | S = 58% | S = 22% | S = 55% | S = 20% | S = 70% | S = 38% |
OX (80 mg/m2) IRI (100 mg/m2) |
| 7 | 9 | S = 53% | S = 55% | S = 35% | S = 85% | S = 35% | S = 55% | S = 23% | S = 45% | S = 20% | MMC (25 mg/m2) |
| 8 | 15.1 | S = 55% | S = 83% | S = 60% | S = 20% | S = 25% | S = 60% | S = 20% | S = 62% | S = 50% | OX (80 mg/m2) |
| 9 | 8.3 | S = 55% | S = 60% | S = 45% | S = 80% | S = 24% | S = 50% | S = 40% | S = 50% | S = 20% | MMC (25 mg/m2) |
| 10 | 8.4 | S = 40% | S = 85% | S = 50% | S = 20% | S = 20% | S = 50% | S = 22% | S = 55% | S = 55% | OX (80 mg/m2) |
| 11 | 8.3 | S = 50% | S = 80% | S = 65% | S = 60% | S = 40% | S = 60% | S = 20% | S = 55% | S = 20% | OX (80 mg/m2) |
| 12 | 8.4 | S = 60% | S = 65% | S = 60% | S = 81% | S = 24% | S = 65% | S = 24% | S = 65% | S = 60% | MMC (25 mg/m2) |
| 13 | 15.2 | S = 60% | S = 60% | S = 40% | S = 85% | S = 20% | S = 60% | S = 45% | S = 50% | S = 20% | MMC (25 mg/m2) |
| 14 | 9.8 | S = 38% | S = 35% | S = 41% | S = 91% | S = 30% | S = 60% | S = 49% | S = 50% | S = 10% | MMC (25 mg/m2) |
| 15 | 6.9 | S = 55% | S = 65% | S = 40% | S = 85% | S = 24% | S = 55% | S = 24% | S = 60% | S = 25% | MMC (25 mg/m2) |
| 16 | 9.7 | S = 60% | S = 62% | S = 65% | S = 83% | S = 65% | S = 68% | S = 22% | S = 65% | S = 60% | MMC (25 mg/m2) |
| 17 | 6.9 | S = 35% | S = 42% | S = 50% | S = 80% | S = 35% | S = 55% | S = 65% | S = 48% | S = 40% | MMC (25 mg/m2) |
| 18 | 9.8 | S = 60% | S = 62% | S = 42% | S = 75% | S = 23% | S = 60% | S = 65% | S = 60% | S = 28% | MMC (25 mg/m2) |
| 19 | 8.4 | S = 50% | S = 75% | S = 50% | S = 80% | S = 20% | S = 45% | S = 35% | S = 60% | S = 70% |
MMC (25 mg/m2) OX (80 mg/m2) RAL (3 mg/m2) |
| 20 | 12.2 | S = 58% | S = 62% | S = 40% | S = 70% | S = 20% | S = 70% | S = 25% | S = 45% | S = 70% |
MMC (25 mg/m2) CIS (70 mg/m2) RAL (3 mg/m2) |
| 21 | 8.3 | S = 25% | S = 80% | S = 70% | S = 80% | S = 24% | S = 40% | S = 20% | S = 42% | S = 20% |
MMC (25 mg/m2) OX (80 mg/m2) IRI (100 mg/m2) |
| 22 | 7.5 | S = 50% | S = 50% | S = 50% | S = 80% | S = 25% | S = 65% | S = 60% | S = 55% | S = 70% |
MMC (25 mg/m2) RAL (3 mg/m2) |
| 23 | 6.9 | S = 25% | S = 55% | S = 45% | S = 80% | S = 30% | S = 50% | S = 65% | S = 45% | S = 75% |
MMC (25 mg/m2) RAL (3 mg/m2) |
| 24 | 15.1 | S = 35% | S = 50% | S = 50% | S = 50% | S = 35% | S = 45% | S = 70% | S = 75% | S = 50% |
ALK (30 mg/m2) CAR (100 mg/m2) |
| 25 | 16.3 | S = 40% | S = 55% | S = 50% | S = 60% | S = 30% | S = 45% | S = 75% | S = 75% | S = 25% |
ALK (30 mg/m2) CAR (100 mg/m2) |
| 26 | 8.9 | S = 45% | S = 70% | S = 20% | S = 25% | S = 20% | S = 60% | S = 30% | S = 60% | S = 10% | OX (80 mg/m2) |
| 27 | 16.2 | S = 31% | S = 65% | S = 20% | S = 52% | S = 72% | S = 41% | S = 25% | S = 55% | S = 20% |
DOX (35 mg/m2) OX (80 mg/m2) |
| 28 | 9.4 | S = 40% | S = 45% | S = 50% | S = 70% | S = 22% | S = 45% | S = 24% | S = 65% | S = 70% |
MMC (25 mg/m2) RAL (3 mg/m2) |
| 29 | 9.8 | S = 50% | S = 50% | S = 45% | S = 70% | S = 24% | S = 40% | S = 60% | S = 50% | S = 70% |
MMC (25 mg/m2) RAL (3 mg/m2) |
| 30 | 10 | S = 45% | S = 70% | S = 45% | S = 75% | S = 20% | S = 60% | S = 50% | S = 45% | S = 30% |
OX (80 mg/m2) MMC (25 mg/m2) |
| 31 | 9.5 | S = 40% | S = 70% | S = 48% | S = 70% | S = 35% | S = 55% | S = 45% | S = 55% | S = 20% |
OX (80 mg/m2) MMC (25 mg/m2) |
| 32 | 8.9 | S = 50% | S = 75% | S = 50% | S = 75% | S = 24% | S = 60% | S = 25% | S = 60% | S = 30% |
OX (80 mg/m2) MMC (25 mg/m2) |
| 33 | 16.2 | S = 45% | S = 60% | S = 45% | S = 80% | S = 35% | S = 60% | S = 30% | S = 65% | S = 25% | MMC (25 mg/m2) |
| 34 | 7.5 | S = 60% | S = 50% | S = 45% | S = 80% | S = 20% | S = 65% | S = 45% | S = 60% | S = 10% | MMC (25 mg/m2) |
| 35 | 8.9 | S = 50% | S = 60% | S = 35% | S = 82% | S = 35% | S = 40% | S = 20% | S = 55% | S = 30% | MMC (25 mg/m2) |
| 36 | 6.9 | S = 60% | S = 60% | S = 50% | S = 75% | S = 20% | S = 55% | S = 25% | S = 45% | S = 10% | MMC (25 mg/m2) |
| 37 | 16.3 | S = 50% | S = 55% | S = 20% | S = 82% | S = 35% | S = 50% | S = 30% | S = 47% | S = 30% | MMC (25 mg/m2) |
| 38 | 8.9 | S = 65% | S = 45% | S = 20% | S = 80% | S = 24% | S = 45% | S = 20% | S = 60% | S = 20% | MMC (25 mg/m2) |
| 39 | 14.8 | S = 50% | S = 55% | S = 30% | S = 75% | S = 35% | S = 50% | S = 20% | S = 50% | S = 30% | MMC (25 mg/m2) |
| 40 | 6.9 | S = 40% | S = 65% | S = 25% | S = 80 | S = 40% | S = 35% | S = 25% | S = 40% | S = 22% | MMC (25 mg/m2) |
| 41 | 9.4 | S = 50% | S = 50% | S = 35% | S = 85% | S = 20% | S = 41% | S = 45% | S = 50% | S = 30% | MMC (25 mg/m2) |
| 42 | 16.2 | S = 50% | S = 60% | S = 35% | S = 80% | S = 20% | S = 50% | S = 23% | S = 60% | S = 30% | MMC (25 mg/m2) |
| 43 | 9.8 | S = 55% | S = 55% | S = 20% | S = 82% | S = 24% | S = 40% | S = 30% | S = 60% | S = 25% | MMC (25 mg/m2) |
| Pt | EGFR | VEGFR | KRAS | NRAS | BRAF | MDR1 (%) | TYMS (%) | DHFR (%) | SHMT1 (%) | ERCC1 (%) | GST (%) | TT |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Part-B | ||||||||||||
| 1 | E = 45% | E = 35% | NM | NM | NM | E = 65% | E = 0% | E = 0% | E = 0% | E = 15% | E = 20% | |
| 2 | E = 45% | E = 45% | NM | NM | NM | E = 60% | E = 10% | E = 0% | E = 0% | E = 20% | E = 20% | |
| 3 | E = 35% | E = 45% | NM | NM | NM | E = 55% | E = 0% | E = 0% | E = 0% | E = 25% | E = 20% | |
| 4 | E = 45% | E = 70% | NM | NM | NM | E = 80% | E = 0% | E = 0% | E = 0% | E = 0% | E = 18% | BEV (5 mg/kg) |
| 5 | E = 45% | E = 70% | NM | NM | NM | E = 80% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 6 | E = 60% | E = 85% | NM | NM | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 7 | E = 60% | E = 55% | NM | NM | NM | E = 60% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | CET (250 mg/m2) |
| 8 | E = 10% | E = 45% | NM | NM | NM | E = 65% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | |
| 9 | E = 20% | E = 20% | NM | NM | NM | E = 70% | E = 10% | E = 0% | E = 0% | E = 0% | E = 10% | |
| 10 | E = 70% | E = 45% | NM | NM | NM | E = 58% | E = 0% | E = 0% | E = 0% | E = 0% | E = 12% | CET (250 mg/m2) |
| 11 | E = 40% | E = 45% | NM | NM | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | |
| 12 | E = 45% | E = 45% | NM | NM | NM | E = 65% | E = 0% | E = 0% | E = 0% | E = 5% | E = 25% | |
| 13 | E = 20% | E = 30% | NM | NM | NM | E = 70% | E = 5% | E = 0% | E = 0% | E = 0% | E = 10% | |
| 14 | E = 20% | E = 20% | MT12e2 | NM | NM | E = 75% | E = 15% | E = 0% | E = 0% | E = 0% | E = 25% | |
| 15 | E = 30% | E = 30% | NM | NM | NM | E = 80% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | |
| 16 | E = 10% | E = 65% | NM | NM | NM | E = 65% | E = 0% | E = 0% | E = 0% | E = 0% | E = 20% | BEV (5 mg/kg) |
| 17 | E = 50% | E = 80% | MM | NM | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 18 | E = 60% | E = 70% | NM | NM | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 19 | E = 50% | E = 65% | NM | NM | NM | E = 65% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 20 | E = 50% | E = 20% | NM | NM | NM | E = 55% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | CET (250 mg/m2) |
| 21 | E = 25% | E = 40% | MT12e2 | NM | NM | E = 80% | E = 10% | E = 0% | E = 0% | E = 20% | E = 20% | |
| 22 | E = 65% | E = 50% | NM | NM | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 0% | E = 5% | CET (250 mg/m2) |
| 23 | E = 50% | E = 80% | NM | NM | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 24 | E = 50% | E = 20% | NM | NM | NM | E = 80% | E = 10% | E = 0% | E = 0% | E = 25% | E = 20% | CET (250 mg/m2) |
| 25 | E = 50% | E = 20% | NM | NM | NM | E = 60% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | CET (250 mg/m2) |
| 26 | E = 50% | E = 30% | NM | NM | NM | E = 65% | E = 10% | E = 0% | E = 0% | E = 0% | E = 10% | CET (250 mg/m2) |
| 27 | E = 80% | E = 35% | NM | NM | NM | E = 60% | E = 0% | E = 25% | E = 0% | E = 0% | E = 0% | CET (250 mg/m2) |
| 28 | E = 40% | E = 35% | MT12e2 | NM | NM | E = 65% | E = 5% | E = 25% | E = 10% | E = 0% | E = 15% | |
| 29 | E = 60% | E = 70% | NM | NM | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 0% | E = 0% | BEV (5 mg/kg) |
| 30 | E = 70% | E = 60% | NM | NM | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 0% | E = 0% | CET (250 mg/m2) |
| 31 | E = 60% | E = 55% | NM | NM | NM | E = 65% | E = 5% | E = 0% | E = 0% | E = 0% | E = 10% | CET (250 mg/m2) |
| 32 | E = 60% | E = 55% | NM | NM | NM | E = 65% | E = 5% | E = 0% | E = 0% | E = 0% | E = 15% | CET (250 mg/m2) |
| 33 | E = 40% | E = 30% | NM | MT61e3 | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 25% | E = 20% | |
| 34 | E = 40% | E = 70% | NM | NM | NM | E = 80% | E = 5% | E = 10% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 35 | E = 50% | E = 70% | NM | NM | NM | E = 70% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 36 | E = 50% | E = 20% | NM | NM | NM | E = 65% | E = 0% | E = 0% | E = 0% | E = 0% | E = 15% | CET (250 mg/m2) |
| 37 | E = 40% | E = 30% | MT13e2 | NM | NM | E = 60% | E = 5% | E = 0% | E = 0% | E = 0% | E = 10% | |
| 38 | E = 40% | E = 80% | NM | NM | NM | E = 70% | E = 5% | E = 0% | E = 0% | E = 15% | E = 15% | BEV (5 mg/kg) |
| 39 | E = 25% | E = 20% | NM | NM | NM | E = 70% | E = 10% | E = 0% | E = 0% | E = 0% | E = 10% | |
| 40 | E = 40% | E = 60% | NM | NM | NM | E = 60% | E = 0% | E = 0% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 41 | E = 50% | E = 70% | NM | NM | NM | E = 70% | E = 10% | E = 0% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 42 | E = 40% | E = 50% | NM | NM | NM | E = 60% | E = 0% | E = 10% | E = 0% | E = 0% | E = 10% | BEV (5 mg/kg) |
| 43 | E = 40% | E = 40% | MT12e2 | NM | NM | E = 65% | E = 0% | E = 0% | E = 0% | E = 0% | E = 5% | |
Pt patient, IV-CTCS isolated viable circulating tumor cells, 5-FU 5 fluorouracil, MMC mitomycin, IRI irinotecan, OX oxaliplatin, RAL raltitrexed, CAR carboplatin, DOX doxorubicin, ALK alkeran, TC tailored chemotherapy, TT target-therapy, S sensitivity, E gene expression, NM no mutations, MT mutated type, 12e2 codon 12 of exon 2, 61e3 codon 61 of exon 3, CET cetuximab, BEV bevacizumab, EGFR epidermal growth factor receptor, VEGFR vascular endothelial growth factor receptor, KRAS Kirsten rat sarcoma virus, NRAS neuroblastoma RAS viral oncogene homolog, BRAF v-Raf murine sarcoma viral oncogene homolog B gene, MDR1 multidrug resistance gene (ABCB1 gene), TYMS thymidylate synthase gene, DHFR dihydrofolate reductase, ERCC1 DNA excision repair protein, GST glutathione S-transferases