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. 2019 Dec 1;33(23-24):1688–1701. doi: 10.1101/gad.331892.119

Figure 5.

Figure 5.

Far-distal regions exhibit variation between acrocentric chromosomes. (A) A schematic illustrating the proposed location of sequences present in BAC clone AL591856 relative to the DJ contig from A9-15 (HSA15). (B) A normal human female (F5) metaphase spread probed with BAC clone AL591856 (green) and alpha satellite probes recognizing HSA13/21 (red) and HSA14/22 (far red, pseudocolored here in blue). Chromosomes were DAPI-stained (pseudocolored in gray). Enlarged versions of all 10 acrocentric p-arms are shown on the right. (C) Human acrocentric chromosomes present in hybrid lines show varying composition of AL591856-like sequences in far-distal regions. The portions of DJ contigs and separate contigs that have high sequence identity with far-distal BAC clone AL591856 are shown below a schematic representation of this BAC. These fall into the same three group classifications described in Figure 3C. Coordinates and perent identities are indicated. The red asterisk beside group 1 member A9-22 indicates the presence of a second contig with lower, but still significant, homology (∼87%) with AL591856 (see Supplemental Fig. S13).