Skip to main content
. Author manuscript; available in PMC: 2020 Jun 21.
Published in final edited form as: ACS Synth Biol. 2019 May 23;8(6):1430–1440. doi: 10.1021/acssynbio.9b00103

Figure 5. Polymerase-mediated synthesis of natural, non-cognate, and xeno-nucleic acid polymers in uniform droplet microcompartments.

Figure 5.

E. coli expressing natural and engineered polymerases were encapsulated in droplet microcompartments, lysed, and assayed for polymerase activity in the presence (left) and absence (right) of the appropriate nucleoside triphosphate. Enzymatic activities include (a) TGO-mediated DNA synthesis; (b) DV-QGLK-mediated RNA synthesis, (c) TGO-WT-mediated FANA synthesis, and (d) KOD-RS-mediated TNA synthesis. The absence of florescence in the no nucleoside triphosphate controls demonstrates that endogenous E. coli nucleotides do not interfere with polymerase activity.