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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2019 Nov 12;146:333–339. doi: 10.1016/j.freeradbiomed.2019.11.014

Figure 3. Respiratory burst assay in isolated peritoneal macrophages from TBI/BMT rats demonstrates the ability of SS-derived hematopoetic cells to produce ROS.

Figure 3.

Luminol derivative L-012 was used as an index of superoxide production. Superoxide production was detected in PMA-stimulated macrophages from isolated from rats received bone marrow from SS donors, which was not evident in macrophages from SSp67phox−/− donors. 1×106 peritoneal macrophages/well, PMA = 135 uM, SOD = 100 U/well. n=6-8. pGroup<0.001 SSp67phox−/− vs SS, ***p<0.001 PMA vs Ctrl (SS), †††p<0.001 PMA vs PMA+SOD (SS).