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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Mol Cancer Res. 2019 Oct 17;18(1):68–78. doi: 10.1158/1541-7786.MCR-19-0187

Figure 4. ELF4 ectopic expression antagonizes the effect of miR-124 on differentiation of neuroblastoma cells.

Figure 4.

(A-B) BE(2)-C cells were first infected with ELF4-expressing lentivirus or control then reverse transfected with miRNA mimics (miR-124 or control). 120 hours later, the impact on differentiation and confluence was measured. (A) Morphology of transfected cells (scale bar = 100 μm). (B) Quantification of neurite outgrowth of treated cells. (C) Confluence of treated cells. (D) Proposed model of ELF4 and miR-124 antagonism. (E) ELF4 and miR-124 expression levels in SK-N-BE(2)-C transfected cells. (F) Expression analysis by qRT-PCR of miR-124 and ELF4-overexpressing shared targets in co-transfected cells were transfected with miRNA mimics; 48 hours later RNA was isolated and qRT-PCR was used to measure expression of a select group of co-targeted genes. Statistical significance of observed changes in neurite outgrowth was determined by a two-way ANOVA with Tukey’s range test for multiple comparisons. A Student’s t-test was used to assess differences in expression. * = p<0.05, **** = p<0.0001.