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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Mol Cancer Res. 2019 Oct 17;18(1):68–78. doi: 10.1158/1541-7786.MCR-19-0187

Figure 5. Synergy between ALK and ELF4 inhibition on NB cell proliferation.

Figure 5.

(A) SH-SY5Y cells were reverse transfected with siRNAs (siALK, siELF4 or control) and their proliferation was monitored with live-cell imaging (IncuCyte). (B) mRNA levels of ALK and ELF4 48 h after transfection. (C) KELLY cells were reverse transfected with siRNAs (siALK, siELF4 or control) and their proliferation was monitored with live-cell imaging. (D) mRNA levels of ALK and ELF4 48 h after transfection. (E) Morphological changes observed. (F) Quantification of the number of viable cells (MTS assay, 120 hours after transfection). (G) Quantification of differentiation (neurite outgrowth, 120 hours after transfection). (H) Proposed model of ALK and ELF4 synergy, based on STRING protein-protein interactions. Statistical significance of observed changes was determined by Student’s t-test with a nominal significance threshold of p = 0.05. * = p<0.05, ** = p<0.01, *** = p<0.001.