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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Mol Cancer Res. 2019 Oct 16;18(1):166–178. doi: 10.1158/1541-7786.MCR-19-0204

Figure 4: ILF2 is a PRMT6 interacting protein.

Figure 4:

A. Mouse lung lysates were employed in affinity pulldowns with either GST- or GST-PRMT6 agarose followed by SDS-PAGE separation. Later, PRMT6-associated proteins were identified by in gel tryptic digestion followed by mass spectrometry. B. STRING protein-protein interaction network of PRMT6 interacting proteins. C. Beas2B cells were transiently transfected with HA-PRMT6 for 48 hours followed by cell lysis and affinity pulldowns with anti-HA antibodies. Interaction of PRMT6 and ILF2 was visualized by probing the immunoblots with anti-ILF2 antibodies. D. His-tagged PRMT6 and GST-tagged ILF2 were purified from E.Coli. Later, equal amounts of His-PRMT6 were mixed with either GST or GST-ILF2 followed by affinity pulldowns with GST-agarose beads. Direct interaction of PRMT6 and ILF2 were visualized by probing the blots with anti-His antibodies. E. In vitro methylation assays were performed by employing HA-PRMT6 purified from Beas2B cell lysates, GST-ILF2 or histone 3 as substrates and 3H-SAMe as a methyl donor. Representative fluorographs were shown in the figure.