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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Adv Mater. 2019 Jul 4;32(13):e1901743. doi: 10.1002/adma.201901743

Figure 6.

Figure 6.

Quencher-free probes. (A) FIT Probes incorporate a viscosity sensitive dye, such that target binding results in turn-on of the dye[124] (B) ECHO probes contain two dyes (e.g. thiazole orange) that are covalently attached to the same base in a probe sequence. In the free probe, the dyes form an H-aggregate resulting in attenuated fluorescence emission. Target binding breaks the H-aggregation, and the dyes intercalate between the bases in the duplex with concomitant fluorescence turn on.[84]